P-161: Effect of Naloxone on Endometrial Growth

Background: There is fine correlation between the infertility and lessening of endometrial thickness. In this study the effects of sedative naloxone, and L-arginine, the precursor of nitric oxide (NO), on endometrial thickness of rat uterus was examined. Materials and Methods: Wistar rats (weighing 200-250 g) in Diestrous phase received intra-peritoneal (i.p.) 1. single L-arginine (50 mg/kg), 2. single naloxone (0.4 mg/ kg) and 3. naloxone (0.4 mg/kg) 30 minutes priorly to the L-arginine (50 mg/kg) during 9 days/ once a day. The control group exclusively received saline (1 ml/kg, i.p.)throughout the treatment period. After completion of the treatments, all rats were coupled with intact males and after observation of vaginal plaques they were isolated and graded 0 of gestation. The females were subsequently passed surgical examination in days 19-20 of gestation. Results: Based on the findings the group that only administered L-arginine, nevertheless of endometrial development, showed low pregnancy and fetal count to control. However, naloxone pre-treatment caused significant raise in the rates. The rate, furthermore, showed an increase in single naloxone group. Conclusion: Endometrial wideness may occur due to angiogenesis and expansion of blood flow in the endometrial wall and by growing of the wall glands and matrix. The L-arginine may produce the NO, a putative increasing factor of blood flow. This factor, however, is not as effective as do in the presence of naloxone. The naloxone can develop the endometrial growth in Wistar rat.